Effects Of Blood Collection And Processing Variables On Hematological Quality Of Packed Red Cells

Dwi Eni Danarsih; Nurpuji Mumpuni

doi:10.31983/jrk.v15i1.14379

Authors

Dwi Eni Danarsih Universitas Jenderal Achmad Yani Yogyakarta
Nurpuji Mumpuni Universitas Jenderal Achmad Yani Yogyakarta

DOI:

https://doi.org/10.31983/jrk.v15i1.14379

Keywords:

Packed Red Cells (PRC) , Blood Collection Time , Blood Processing Method , Hematological Quality , Blood Transfusion Safety

Abstract

This study aimed to analyze the relationship between blood bag type, blood collection duration, and processing time with the quality of Packed Red Cells (PRC) to support transfusion safety. An observational analytic study with a cross-sectional design was conducted at the Blood Transfusion Unit of PMI Bantul. A total of 50 PRC units collected from male voluntary donors were analyzed. Variables included blood collection duration, processing delay, blood bag type, and processing method, while PRC quality was assessed based on hemoglobin concentration, hematocrit, and physical appearance. Data were analyzed using Spearman correlation and Mann–Whitney tests. The results showed that blood collection duration and processing delay had no significant effect on PRC hemoglobin levels. However, both factors demonstrated a significant negative correlation with PRC hematocrit, indicating that longer collection and delayed processing reduced hematocrit values. Processing method significantly affected hematocrit, with centrifugation producing higher hematocrit compared to sedimentation, while blood bag type showed no significant influence. All PRC units met quality standards with clear plasma and no clot formation. This study highlights the importance of timely blood processing and appropriate processing methods to maintain PRC quality, thereby contributing to improved transfusion safety and blood bank quality management.

References

Aran, G., Torrents, S., Blanco, M., Somarriba, N., Algar, F. J., Shettiyar, F., Galvez, L., Lopez, S., Amill, B., Lloret, M., Torramade-Moix, S., Martinez-Llonch, N., Soria, G., & Codinach, M. (2026). Automated cell count for blood component quality control. Transfusion. https://doi.org/10.1111/trf.70101

Astuti, Y., & Artini, D. (2019). COMPARATIVE HEMOGLOBIN AND HEMATOCRIT BEFORE AND AFTER DONATION TO BLOOD DONATE IN UNIT TRANSFUSION YOGYAKARTA CITY. Jurnal Riset Kesehatan, 8(2), 40–42. https://doi.org/10.31983/jrk.v8i2.5362

Astuti, Y., Dewi, Y. L. R., & Murti, B. (2025). The Effect Of Blood Transfusion On The Risk Of Hepatitis B Infection In Hemodialysis Patients : Meta Analysis. Jurnal Riset Kesehatan, 14(1), 17–22. https://doi.org/10.31983/jrk.v14i1.9342

Astutiningtiyas, S., Rusyadi, L., & Kartikasari, Y. (2022). QUALITY CONTROL OF PACKED RED CELL (PRC) PRODUCT IN BLOOD DONATION UNIT. Jurnal Riset Kesehatan, 11(1), 21–27. https://doi.org/10.31983/jrk.v11i1.8506

Cahyani, S., & Ihtiaringtyas, S. (2020). Karakteristik Pendonor Darah yang Gagal Pengambilan Darah. Jurnal Ilmiah Cerebral Medika, Vol. 2, No. 2.

Danarsih, D. E., Artini, D., & Purnamaningsih, N. (2023). Pengaruh Obesitas Terhadap Kadar Hemoglobin dan Hematokrit Sampel Darah Pendonor Selama Penyimpanan 35 Hari. 16(September 2023).

Danarsih, D. E., Kusumawardani, A. M., & Ariningtyas, R. E. (2023). Hubungan Antara Indeks Massa Tubuh dan Kadar Hemoglobin pada Remaja Putri. Jurnal Indonesia Sehat, 2(2), 53–58.

Hanifah, A. Y., Putri, D. E., & Widada, N. S. (2022). PERBEDAAN KADAR HEMOGLOBIN PADA KOMPONEN PRC SIMPAN DENGAN INTERVAL PENYIMPANAN 7 HARI DI BANK DARAH RSUD BUDHI ASIH. 4(3).

Harisanti, S., Arman, E., & Idaman, M. (2021). The Effect of Storage Duration on Hemoglobin and Hematocrit Levels of Packed Red Cell (PRC) Products.

Made, N., Dwijastuti, S., Ketut, I., Damarta, A., & Widiantari, N. P. (2023). THE DIFFERENCES OF HEMATOCRIT LEVEL IN IMMEDIATE AND DELAYED BLOOD SAMPLES ON MICROHEMATOCRIT METHOD. In The Journal Of Medical Laboratory) Meditory | e (Vol. 11, Number 2).

Maurer, F., John, T., Makhro, A., Bogdanova, A., Minetti, G., Wagner, C., & Kaestner, L. (2022). Continuous Percoll Gradient Centrifugation of Erythrocytes-Explanation of Cellular Bands and Compromised Age Separation. Cells, 11(8). https://doi.org/10.3390/cells11081296

Nelma, & Adiratna, N. M. (2023). Pengaruh Penyimpanan Darah Terhadap Kadar Hemoglobin pada Komponen Whole Blood Darah Donor Sebelum dan Sesudah Disimpan Selama Satu Minggu di PMI Kota Medan. JoIMedLabS, 4(1), 70–77.

Permenkes No. 91, 2015. (2015). PERATURAN MENTERI KESEHATAN REPUBLIK INDONESIA NOMOR 91 TAHUN 2015 TENTANG STANDART PELAYANAN TRANSFUSI DARAH. Ekp, 13(3), 1576–1580.

Permenkes RI. (2015a). Permenkes Ri Nomor 91 Tahun 2015. Standar Pelayanan Transfusi Darah, 16(1994), 1–27.

Permenkes RI. (2015b). Permenkes Ri Nomor 91 Tahun 2015. Standar Pelayanan Transfusi Darah, 16(1994), 1–27.

https://promkes.kemkes.go.id/phbs%0Ahttp://eprints.ums.ac.id/37501/6/BAB II.pdf

Quirynen, M., Siawasch, S. A. M., Yu, J., & Miron, R. J. (2025). Essential principles for blood centrifugation. In Periodontology 2000 (Vol. 97, Number 1, pp. 43–51). John Wiley and Sons Inc. https://doi.org/10.1111/prd.12555

Sawadogo, S., Moindze, A., Nebie, Koumpingnin, Kafando, A., Sawadogo, A.-G., Ouili, P., & Kafando, E. (2021). Evaluation of hemolysis during storage of red blood cell concentrates processed by centrifugation and settling method by simple gravity in Burkina Faso. Hematology & Transfusion International Journal, 9(3), 57–61. https://doi.org/10.15406/htij.2021.09.00253

Sepvianti, W., Wulandari, M., Btari Christiyani Kusumaningrum, S., Sunartono, & Djafar, T. (2019). Gambaran Kadar Hemoglobin pada Sediaan Produk Darah Packed Red Cells (PRC) selama Masa Simpan 20 hari. Journal of Health, 5(2), 123–125.

Subekti, Wijaya, G. H., Anna, K. Y. A., & Widoyono. (2024). Pengaruh Durasi Penyimpanan Terhadap Kualitas Packed Red Cell Di UDD PMI Kota Semarang Effect of Storage Duration on the Quality of Packed Red Cells at UDD PMI Semarang City. Medika Trada : Jurnal Teknik Elektromedik Polbitrada, 5(1).

Syuhada, Aditya, & Candrawijaya, I. (2020). Perbedaan Hematokrit Darah Segar dan Darah Simpan (30 Hari) di UTD RSAM Bandar Lampung. 9(2). https://doi.org/10.35816/jiskh.v10i2.379

van der Meer, P. F., & de Korte, D. (2015). The Effect of Holding Times of Whole Blood and Its Components During Processing on In Vitro and In Vivo Quality. In Transfusion Medicine Reviews (Vol. 29, Number 1, pp. 24–34). W.B. Saunders. https://doi.org/10.1016/j.tmrv.2014.10.001

World Health Organization (WHO). (2025). Annex 4 Good practices for blood establishments Replacement of Annex 4 of WHO Technical Report Series, No. 961.

Yanti, O. W., & Astuti, T. dyah. (2025). Perbandingan kadar Eritrosit dan Hematokrit Darah EDTA yang segera Diperiksa dan Ditunda Selama 1, 2, dan 3 jam. Jurnal Kesehatan Tambusai, (1).

Zhang, S., Li, G., Wang, J., Wang, D., Han, Y., Cao, H., & Lin, L. (2018). Nondestructive Measurement of Hemoglobin in Blood Bags Based on Multi-Pathlength VIS-NIR Spectroscopy. Scientific Reports, 8(1). https://doi.org/