COMPARATIVE EXAMINATION OF CONVENTIONAL DIRECT SPUTUM AND INDIRECT SEDIMENTATION ON CYTOSPIN TUBERCULOSIS PATIENT SPUTUM SAMPLES
Abstract
Microscopic examination of sputum using the Ziehl-Neelsen stain is the gold standard for Tuberculosis (TB), but it must be performed by experts with special skills. The purpose of this study is to accelerate the determination of the microscopic results using the Ziehl-Neelsen stain. This research is an experimental method in which the test sample is treated and the sputum sample is controlled with up to 25 samples. The method development is very important to increase the sensitivity and specificity of the results of TB examination using microscopes. This observation shows that the indirect cytospin method has a narrower reading range on a circle with a diameter of only 7 mm, making it easier for a bacterial count compared to the traditional direct method with a size of 2 x 3 cm oval shape. The results of the microscopic examination were 21 positive specimens and 4 negative specimens. Mycobacterium tuberculosis with Ziehl-Neelsen staining gave the same results with a sensitivity and specificity of 100%.
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Agrawal, M., Bajaj, A., Bhatia, V., & Dutt, S. (2016). Comparative study of GeneXpert with ZN stain and culture in samples of suspected pulmonary tuberculosis. Journal of Clinical and Diagnostic Research, 10(5), DC09-DC12. https://doi.org/10.7860/JCDR/2016/18837.7755
Ahmad, Mujeeb, Stanikzai, M. H., Rahimy, N., Wasiq, A. W., & Sayam, H. (2021). Diagnostic Accuracy of Ziehl-Neelsen Smear Microscopy in Comparison with GeneXpert in Pulmonary Tuberculosis: A Multi-Center Study in Kandahar Province, Afghanistan. Asian Journal of Medicine and Health, (March), 1–7. https://doi.org/10.9734/ajmah/2021/v19i230299
Ahmad, Mushtaq, Ibrahim, W. H., Sarafandi, S. Al, Shahzada, K. S.,
Ahmed, S., Haq, I. U., … Sattar, H. A. (2019). Diagnostic value of bronchoalveolar lavage in the subset of patients with negative sputum/smear and mycobacterial culture and suspicion of pulmonary tuberculosis. International Journal of Infectious Diseases, 82, 96–101. https://doi.org/10.1016/j.ijid.2019.03.021
Angaali, N., Apparao Patil, M., & Dharma Teja, V. (2019). Detection of Mycobacterium tuberculosis - Microscopy to Molecular Techniques at the Tertiary Care Hospital in Telangana. Iranian Journal of Medical Microbiology, 13(3), 175–179. https://doi.org/10.30699/ijmm.13.3.175
Anthwal, D., Gupta, R. K., Gomathi, N. S., Tripathy, S. P., Das, D., Pati, S., … Tyagi, J. S. (2021). Evaluation of ‘TBDetect’ sputum microscopy kit for improved detection of Mycobacterium tuberculosis: a multi-centric validation study. Clinical Microbiology and Infection, 27(6), 911.e1-911.e7. https://doi.org/10.1016/j.cmi.2020.08.020
Chen, P., Shi, M., Feng, G. D., Liu, J. Y., Wang, B. J., Shi, X. D., … Zhao, G. (2012). A highly efficient Ziehl-Neelsen stain: Identifying de novo intracellular Mycobacterium tuberculosis and improving detection of extracellular M. tuberculosis in cerebrospinal fluid. Journal of Clinical Microbiology, 50(4), 1166–1170. https://doi.org/10.1128/JCM.05756-11
Fennelly, K. P., Morais, C. G., Hadad, D. J., Vinhas, S., Dietze, R., & Palacib, M. (2012). The small membrane filter method of microscopy to diagnose pulmonary tuberculosis. Journal of Clinical Microbiology, 50(6), 2096–2099. https://doi.org/10.1128/JCM.00572-12
Fouda, M. E., Gwad, E. R. A., Fayed, S. M., Kamel, M. H., & Ahmed, S. A. (2019). Egyptian Journal of Medical Microbiology A Study of the Added Value of Xpert MTB / RIF Assay for Assessment of Pulmonary Tuberculosis Transmission Risk ORIGINAL ARTICLE A Study of the Added Value of Xpert MTB / RIF Assay for Assessment of Pulmonary Tuberculosis Transmission Risk. (September).
G., A. K., Chandrasekaran, S., J., V., & B., V. K. (2017). Bleach method in comparison with NALC-NaOH specimen processing method for the detection of mycobacterium in sputum specimen. International Journal of Research in Medical Sciences, 5(9), 3865. https://doi.org/10.18203/2320-6012.ijrms20173645
Heemskerk, A. D., Donovan, J., Thu, D. D. A., Marais, S., Chaidir, L., Dung, V. T. M., … Thwaites, G. E. (2018). Improving the microbiological diagnosis of tuberculous meningitis: A prospective, international, multicentre comparison of conventional and modified Ziehl–Neelsen stain, GeneXpert, and culture of cerebrospinal fluid. Journal of Infection, 77(6), 509–515. https://doi.org/10.1016/j.jinf.2018.09.003
Ibrahim, A. U., Guler, E., Guvenir, M., Suer, K., Serte, S., & Ozsoz, M. (2021). Automated detection of Mycobacterium tuberculosis using transfer learning. Journal of Infection in Developing Countries, 15(5), 678–686. https://doi.org/10.3855/JIDC.13532
Imtiaz, A., Ikram, A., Zaman, G., Satti, L., & Sana, F. (2021). E v a l u a t i o n o f m o d i f i e d c y t o s p i n s l i d e m i c r o s c o p y f o r d e t e c t i o n o f a c i d f a s t b a c i l l i i n b r o n c h o a l v e o l a r l a v a g e f l u i d f o r d i a g n o s i s o f p u l m o n a r y t u b e r. 71(3), 920–923.
Khutlang, R., Krishnan, S., Dendere, R., Whitelaw, A., Veropoulos, K., Learmonth, G., & Douglas, T. S. (2010). Classification of mycobacterium tuberculosis in images of ZN-stained sputum smears. IEEE Transactions on Information Technology in Biomedicine, 14(4), 949–957. https://doi.org/10.1109/TITB.2009.2028339
Kurmi, Y., Chaurasia, V., Goel, A., Joshi, D., & Kapoor, N. (2021). Tuberculosis bacteria analysis in acid-fast stained images of sputum smear. Signal, Image and Video Processing, 15(1), 175–183. https://doi.org/10.1007/s11760-020-01732-1
Ludam, R., & Jena, B. (2019). Microscopic versus culture methods for diagnosis of Mycobacterium tuberculosis: Our experience. International Journal of Advanced Research in Medicine, 1(2), 109–111. https://doi.org/10.22271/27069567.2019.v1.i2b.93
MacLean, E., Bigio, J., Singh, U., Klinton, J. S., & Pai, M. (2021). Global tuberculosis awards must do better with equity, diversity, and inclusion. The Lancet, 397(10270), 192–193. https://doi.org/10.1016/S0140-6736(20)32627-1
Magalhães, J. L. de O., Lima, J. F. da C., de Araújo, A. A., Coutinho, I. O., Leal, N. C., & de Almeida, A. M. P. (2018). Microscopic detection of mycobacterium tuberculosis in direct or processed sputum smears. Revista Da Sociedade Brasileira de Medicina Tropical, 51(2), 237–239. https://doi.org/10.1590/0037-8682-0238-2017
Mindolli, P. B., Salmani, M. P., & Parandekar, P. K. (2013). Improved diagnosis of pulmonary tuberculosis using bleach microscopy method. Journal of Clinical and Diagnostic Research, 7(7), 1336–1338. https://doi.org/10.7860/JCDR/2013/6152.3189
Roy, R. Das, & Gupta, S. Das. (2022). A Comparative Study of Cartridge ‑ Based Nucleic Acid Amplification Test and Ziehl – Neelsen Stain with Culture on Lowenstein – Jensen Media as Gold Standard for the Diagnosis of Pulmonary Tuberculosis. 39–42. https://doi.org/10.4103/ijrc.ijrc
State, O. (2021). Efficacy of Fluorescence Light Emitting Diode ( LED ) Microscopy for Detection of Mycobacterium Tuberculosis in Sputum of Patients Attending a Tertiary Hospital in Osogbo. 3(1).
Storch, G. A. (2018). Chapter 170 - Diagnostic Microbiology. In Nelson Textbook of Pediatrics, 2-Volume Set (Twentieth). Elsevier Inc. https://doi.org/10.1016/B978-1-4557-7566-8.00170-8
Uddin, M. K. M., Chowdhury, M. R., Ahmed, S., Rahman, M. T., Khatun, R., Van Leth, F., & Banu, S. (2013). Comparison of direct versus concentrated smear microscopy in detection of pulmonary tuberculosis. BMC Research Notes, 6(1). https://doi.org/10.1186/1756-0500-6-291
WHO. (2020). Tuberculosis Reports. In The Lancet (Vol. 188). https://doi.org/10.1016/S0140-6736(00)58733-9
Widodo, W., Irianto, A., & Pramono, H. (2017). Karakteristik Morfologi Mycobacterium tuberculosis yang Terpapar Obat Anti TB Isoniazid (INH) secara Morfologi. Biosfera, 33(3), 109. https://doi.org/10.20884/1.mib.2016.33.3.316
Widodo, W., & Priyatno, D. (2020). DETECTION OF THE RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS FROM SPECIMENS WITH TB PATIENTS IN SEMARANG BALKESMAS. Jurnal Riset Kesehatan. https://doi.org/10.31983/jrk.v9i1.5583
Zheng, L. H., Jia, H. Y., Liu, X. J., Sun, H. S., Du, F. J., Pan, L. P., … Zhang, Z. D. (2016). Modified cytospin slide microscopy method for rapid diagnosis of smear-negative pulmonary tuberculosis. International Journal of Tuberculosis and Lung Disease, 20(4), 456–461. https://doi.org/10.5588/ijtld.15.0733
Zingue, D., Weber, P., Soltani, F., Raoult, D., & Drancourt, M. (2018). Automatic microscopic detection of mycobacteria in sputum: a proof-of-concept. Scientific Reports, 8(1), 1–6. https://doi.org/10.1038/s41598-018-29660-8
DOI: https://doi.org/10.31983/jrk.v11i1.8406
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