ACCURATE DETECTION OF VIRAL SEROTYPE DENGUE HEMORRHAGIC FEVER THROUGH AEDES SP MOSQUITOES USING REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR)
Abstract
Dengue Hemorrhagic Fever (DHF) caused Dengue virus which transmitted through the bite of the Aedes sp. Detection of dengue virus serotypes is essential for epidemiology as well as potential disease pathogens. RT-PCR method was more effective in mosquitoes, because the virus contained in the mosquito body is passed on to the next generation (trans ovarian). The purpose of this research is to know the serotype of DHF virus accurately through vector mosquito using RT-PCR method in Mataram City, so that vector control, early diagnosis and management of DHF disease could be done quickly and precisely. This research was a laboratory explorative research with cross sectional design that determines serotype of DHF virus through vector mosquito using RT-PCR method in Mataram City. The population of this study were Aedes aegypti female mosquitoes from eggs. Collected from houses located in Community Health Center Mataram city worked area with high dengue cases: Pagesangan, Karang Taliwang, Karang Pule, Tanjung Karang, Mataram, and Selaparang especially around the house’s sufferers. The results showed that only the mosquito group from the worked area of the Mataram Community Health Center showed the ribbon image with the base pair in accordance with serotype 1 (DENGUE 1), the other negative.
Keywords
Full Text:
PDFReferences
Andriyoko, B., Parwati, I., Tjandrawati, A., & Lismayanti, L. (2011). Penentuan Serotipe Virus Dengue dan Gambaran Manifestasi Klinis serta Hematologi Rutin pada Infeksi Virus Dengue Dengue Virus Serotyping and Its Clinical Manifestation and Routine Haematology in Dengue Infections. Mkb, 44(4), 253–260. http://journal.fk.unpad.ac.id/index.php/mkb/article/view/138/pdf_42
Dikes, S. P. B., & Mataram, K. (2016). Perkembangan Sebaran Kasus DBD di Kota Mataram.
Dinkes NTB. (2015). Profil Dinas Kesehatan Provinsi Nusa Tenggara Barat. http://www.depkes.go.id/
Genis, G. (2007). Demam Berdarah - Dr Genis Ginanjar - google books.
Hadinegoro, S. R. H. (2004). Tata Laksana DBD (3rd ed.). DepKes RI. https://silahuddinm.files.wordpress.com/2013/02/bk2007-g4.pdf
Hasmiwati, Dahelmi, and N. (2009). Deteksi Virus Dengue dari Nyamuk Vektor Aedes aegypti di Daerah Endemik Demam Berdarah Dengue (DBD) di Kota Padang dengan Metode Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). In Gudang Penyimpanan Data Ilmiah Universitas Andalas. http://repository.unand.ac.id/id/eprint/739
Inayati, N., Supargiyono, & Umniyati, S. R. (2011). The Differences of the Prevalences and Serotypes of Dengue Virus on Aedes Aegypti Mosquitoes from Pagutan and PagutanTimur in the Sub District of Mataram. Tmj, 02(1), 1–11.
InfoDatin Kementerian Kesehatan. (2016). Situasi DBD di Indonesia. InfoDATIN. https://doi.org/ISSN 2442-7659
Joshi V1, Mourya DT, S. R. (2002). Persistence of dengue-3 virus through transovaria mosquitoes. Am J Trop Med Hyg. 2002 Aug;67(2):158-61., Aug;67(2), 158-61.
Karyanti, M. R., & Hadinegoro, S. R. (2016). Perubahan Epidemiologi Demam Berdarah Dengue Di Indonesia. Sari Pediatri, 10(6), 424. https://doi.org/10.14238/sp10.6.2009.424-32
Mashoedi, I. D. (2007). Hubungan antara Distribusi Serotipe Virus Dengue dari Isolat Nyamuk Aedes spesies dengan Tingkat Endemisitas Demam Berdarah Dengue: Studi Kasus di Kota Semarang [Universitas Diponegoro Semarang]. In Epidemiology. http://eprints.undip.ac.id/5258/1/Imam_Djamaluddin_Mashoedi.pdf
Masrochah, S., Susanto, E., & Irmawati, I. (2017). Sistem Informasi Pemantauan Kejadian Luar Biasa (Klb) Demam Berdarah Berbasis Geographic Information System (Gis) Di Kota Semarang. Jurnal Riset Kesehatan, 5(2), 53. https://doi.org/10.31983/jrk.v5i2.798
Nurfadly. (2009). Deteksi dan penentuan serotipe virus dengue tipe 1 dari nyamuk Aedes aegypti dengan menggunakan Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) di Kota Medan. In USU Repository.
Perich MJ1, Kardec A, Braga IA, Portal IF, Burge R, Zeichner BC, Brogdon WA, W. R. (2003). Field evaluation of a lethal ovitrap against dengue vectors in Brazil. Med Vet Entomol, 17 (2), 205–210. https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2915.2003.00427.x
Ridwan M Fauzi (Republika). (2016, April 19). Ada 426 Kasus DBD di Mataram , Wali Kota. April, 2016.
Thavara, U., Siriyasatien, P., Tawatsin, A., Asavadachanukorn, P., Anantapreecha, S., Wongwanich, R., & Mulla, M. S. (2006). Double infection of heteroserotypes of dengue viruses in field populations of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) and serological features of dengue viruses found in patients in southern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 37(3), 468–476.
Trovancia, G., Sorisi, A., & Tuda, J. S. B. (2016). Deteksi transmisi virus dengue pada nyamuk wild Aedes Aegypti betina di Kota Manado. Jurnal E-Biomedik (EBM), 4(2).
Wahyuni, C., Keman, S., & Fathi, F. (2005). Peran Faktor Lingkungan dan Perilaku terhadap Penularan Demam Berdarah Dengue di Kota Mataram. Jurnal Kesehatan Lingkungan Unair, 2(1), 3944.
WHO. (2011). Comprehensive guidelines for prevention and control of dengue and dengue haemorrhagic fever. In WHO Regional Publication SEARO. https://doi.org/10.1017/CBO9781107415324.004
Yong, Y. K., Thayan, R., Chong, H. T., Tan, C. T., & Sekaran, S. D. (2007). Rapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR. Singapore Medical Journal, 48(7), 662–668. http://www.ncbi.nlm.nih.gov/pubmed/17609830
Yudhastuti, R., & Vidiyani, A. (2005). Hubungan Kondisi Lingkungan, Kontainer, Dan Perilaku Masyarakat Dengan Keberadaan Jentik Nyamuk Aedes Aegypti di Daerah Endemis Demam Berdarah Dengue Surabaya. Jurnal Kesehatan Lingkungan Unair, 1(2), 3942. https://www.mendeley.com/catalogue/d2787957-a1ce-3d8f-977e-ccb2f3853b2d/?utm_source=desktop&utm_medium=1.19.4&utm_campaign=open_catalog&userDocumentId=%7B3338be6d-1dfe-38ea-8fd0-5b881a0cd6d9%7D%0D
Yustin Tatontos, E. (2008). Uji Resistensi Larva Aedes sp dari daerah Endemis Demam Berdarah Dengue Kota Mataram dan Larva Aedes aegypti Koloni Lembaga Penyakit Tropis Universitas Airlangga Surabaya terhadap temefos [Airlangga, surabaya]. https://fk.unair.ac.id/archives/2008/08/10/uji-resistensi-larva-aedes-sp-dari-daerah-endemis-demam-berdarah-dengue-kota-mataram-dan-larva-aedes-aegypti-koloni-lembaga-penyakit-tropis-universitas-airlangga-surabaya.html
DOI: https://doi.org/10.31983/jrk.v10i2.7706
Article Metrics
Refbacks
- There are currently no refbacks.
Copyright (c) 2021 Jurnal Riset Kesehatan
INDEXING
RECOMMENDED TOOLS
JURNAL
RISET
KESEHATAN
Commitment to quality
Double-blind peer review. Immediate open access. Authors retain copyright. Author fee Rp 500.000,00. Published biannually.
Journal Riset Kesehatan (JRK), its website, and the articles published therein are licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
WGA
StatCounter
Google Analytics