Jaringan Laboratorium Medis

Vol. 8 No. 2 (2026): November 2026
Original Articles

Potential of Coffee Leaves (Coffea canaphora) Pengaron from South Kalimantan as an Antibacterial against Staphylococcus epidermidis and Pseudomonas aeruginosa

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  • Nurbidayah,
  • Nadya Al Mayda,
  • Nafila,
  • Rizka Ayu Wahyuni
Nurbidayah
UNBL
Nadya Al Mayda
Universitas Borneo Lestari
Nafila
Universitas Borneo Lestari
Rizka Ayu Wahyuni
Universitas Borneo Lestari
DOI: https://doi.org/10.31983/jlm.v8i2.14779

Published 2026-10-01

Keywords

  • Coffea canephora,
  • antibacterial,
  • Staphylococcus epidermidis,
  • Pseudomonas aeruginosa

How to Cite

Nurbidayah, Al Mayda, N., Nafila, & Ayu Wahyuni, R. (2026). Potential of Coffee Leaves (Coffea canaphora) Pengaron from South Kalimantan as an Antibacterial against Staphylococcus epidermidis and Pseudomonas aeruginosa. Jaringan Laboratorium Medis, 8(2), 101–109. https://doi.org/10.31983/jlm.v8i2.14779

Copyright (c) 2026 Jaringan Laboratorium Medis

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Abstract

Infection is a condition in which microorganisms enter and develop in the body, potentially disrupting normal functions and causing disease. One pathogenic bacterium that causes skin infections is Pseudomonas aeruginosa, while Staphylococcus epidermidis is a normal flora that may act opportunistically. The increasing problem of antibiotic resistance has encouraged research on natural antibacterial sources, one of which is robusta coffee leaves (Coffea canephora), known to contain secondary metabolites such as alkaloids, flavonoids, saponins, and tannins. This study aimed to evaluate the antibacterial activity of 96% ethanol extract of robusta coffee leaves (Pengaron variety) against P. aeruginosa and S. epidermidis. The method used was a quasi-experimental design with a post-test only control group. Treatments consisted of four extract concentrations (5%, 10%, 15%, and 20%), a positive control (Gentamicin disk 10 µg), and a negative control (sterile distilled water). Antibacterial activity was tested using the well diffusion method with four replications. The results showed that robusta coffee leaf extract did not inhibit the growth of P. aeruginosa. In contrast, against S. epidermidis, inhibition zones were observed with moderate activity at 5% concentration (7 mm) and strong activity at 10% (10.6 mm), 15% (13 mm), and 20% (14.5 mm). In conclusion, the 96% ethanol extract of robusta coffee leaves has antibacterial potential against Staphylococcus epidermidis but is not effective against Pseudomonas aeruginosa.

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