Microscopic Profile of Mice (Mus Musculus) Kidney Tissue Fixed with 10% Honey Concentration for 24 Hours

Tasya Vita Brilian

Abstract


Fixation is used to maintain tissue structure in its original form “life-like state” and can protect proteins and tissue components from degeneration. The solution commonly used is 10% NBF. Formaldehyde is chemical substance that is toxic and not environmentally friendly, several studies have shown alternative substitutes fixation, one of which is the honey solution. The study of Mohammed et al (2020) fixated tissue with honey 10% and 20% shown good coloring properties and similar clarity to fixated with formalin 10%. Honey has acidic and dehydrating properties allow most microorganisms to be killed so that tissues will last for a long time. The research objective is to findout the description of microscopic of mice (Mus musculus) kidney tissue which were fixation using 10% honey solution for 24 hours. The research is included in qualitative descriptive research. The research design used was a non-eksperimental with a purposive sampling study approach. The sample used was 32 preparation with total of microscopic overview is 160. Microscopic image of mice (Mus musculus) kidney tissue fixed using 10% honey solution for 24 hours in 80 visual fields were 12.5% of the preparations is not good and 87.5% is good preparations. The microscopic image of mice (Mus musculus) kidney tissue fixed using 10% NBF is better than of the microscopic image of mice (Mus musculus) kidney tissue fixed with 10% honey for 24 hours.


Keywords


Microscopic Overview ; Fixation ; Kidney of Mice

References


Abubakar et al (2020). Efficacy of Various Concentrations of Honey Solution in the Fixation of Some Selected Tissues in Wistar Rats. Advances in Research, 21(7), 34–40.

Afrida, A. D., & Priyatno, D. (2021). Gambaran Histologi Jaringan Hepar Mencit ( Mus Musculus ) yang Difiksasi dengan Larutan Carnoy dengan Variasi Waktu 4 Jam , 8 Jam dan 12 Jam. Jaringan Laboratorium Medis, 03(01), 37–42.

Al-Maaini, R., & Bryant, P. (2006). The effectiveness of honey as a substitute for formalin in the histological fixation of tissue. Journal of Histotechnology, 29(3), 173–176.

Fajrina S, Ariyadi., & Nuroini, F. (2018). Gambaran Kualitas Sediaan Jaringan Hati Menggunakan Larutan Fiksatif NBF 10% dan Alkohol 70% Pada Pewarnaan HE (Hematoksilin-Eosin). In Prosiding Seminar Nasional Mahasiswa Universitas Muhammadiyah Semarang, 1 (1).

I. Udonkang, M., A Ubi, K., & J Inyang, I. (2018). Honey As Fixative and Safer Substitute for Formalin in Histology. International Journal of Medical Laboratory Research, 03(03), 11–17.

Khristian, E, dan Inderiati, D. (2017). Bahan Ajar Teknologi Laboratorium Medis Sitohistoteknologi. Jakarta : Pusat Pendidikan Sumber Daya Manusia Kesehatan Badan Pengembangan dan Pemberdayaan Sumber Daya Kesehatan Manusia.

Miranti, I. P. (2010). Pengolahan Jaringan Untuk Penelitian Hewan Coba. Semarang : Universitas Diponegoro.

Özkan, N., Şalva, E., Çakalaǧaoǧlu, F., & Tüzüner, B. (2012). Honey as a substitute for formalin? Biotechnic and Histochemistry, 87(2), 148–153.

Pratiwi, E. N., & Armalina, D. (2021). Mikroskopis Preparat Mus Musculus Ginjal Dideparafinisasi dengan Minyak Zaitun pada Pewarnaan Eosin ( HE ) Hematoxylin ( HE ). Jaringan Laboratorium Medis, 03(01), 61–66.

Purjanto, K. A., Widiyanto, S. Y. D., & Budiharjo, T. (2019). The Study ofThe Type Laboratory Examination in Health Service Facilities With Integration Determination of Local Content Courses DIII-Health Analyst. Jaringan Laboratorium Medis, 1(2), 77–8

Rosyadi, Akhmad Imron (2020). Gambaran Makroskopis dan Mikroskopis Histologi Hepar Mencit (Mus musculus) yang Difiksasi Menggunakan NBF 10% dan Madu Konsentrasi 10%, 15% dan 20%. Karya Tulis Ilmiah, Poltekkes Kemenkes Semarang.

Slaoui, M., & Fiette, L. (2011). Histopathology Procedures: From Tissue Sampling To Histopathological Evaluation. Methods in Molecular Biology (Clifton, N.J.), 69 (1), 69–82.

Tri Harjana, Mp. (2011). Buku ajar Histologi. Jurusan Pendidikan Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam : Universitas Negeri Yogyakarta.

Udonkang, M., A Ubi, K., & J Inyang, I. (2018). Honey As Fixative and Safer Substitute for Formalin in Histology. International Journal of Medical Laboratory Research, 03(03), 11–17.




DOI: https://doi.org/10.31983/jlm.v3i2.8066

Article Metrics

Abstract view : 186
Download PDF (Bahasa Indonesia) : 283

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Jaringan Laboratorium Medis

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

--

OFFICE INFORMATION :

Jurusan Analis Kesehatan - Politeknik Kesehatan Kemenkes Semarang, http://analis.poltekkes-smg.ac.id Jl. Wolter Monginsidi No. 115 Pedurungan Tengah, Semarang, Jawa Tengah, Indonesia ; Telp./Fax: (024)6723014 Email: jlm@poltekkes-smg.ac.id

Statcounter  Lisensi Creative Commons Jaringan Laboratorium Medis disseminated below Creative Commons Attribution-ShareAlike 4.0 International License.

Our Related Accounts :

 

  
MoU PATELKIISSN LIPIGoogle ScholarGarudaStat CounterDOI Crossref  

 

Jaringan Laboratorium Medis © 2019