Degree of agglutination of blood group examination Cell Celling Method Based on Cell Suspension Concentration Level

Nur Muhjah Khoolidah, Nurul Qomariyah

Abstract


Blood transfusions must go through of strict procedures to prevent transfusion reactions that can appeared. One  procedure that must be do is the examination of ABO and Rhesus blood group. Ratio of antigens and antibodies play a role in the formation of agglutination. The more bonded antigens-antibodies, the larger, clearer, and stronger the agglutination will be. Analyzing the different of degree of agglutination of blood examination cell grouping method based on cell suspension concentration level. The type of research used is experimental research. The subjects of the study were 18 college students were grouped by blood group A, B and AB. Each cell specimen was treated to make cell suspension concentration of 5%, 10%, 15% and 20%. All examination result show degree of agglutination 4+. The cell suspension concentration of 5%, 10%, 15% and 20% in blood type A and B examination show the same data that’s degree of agglutination 4+ with clear background. The cell suspension concentration of 5 in blood type AB examination show degree of agglutination 4+ with clear background of 70%.


Keywords


Blood Examination ; Cell Grouping ; Cell Suspension Concentration ; Degree of Agglutination

References


Bakta, I Made. 2012. Hematologi Klinik Ringkas. Jakarta: EGC.

Bima, Ariotejo. 2014. Laporan Pelatihan dokter baru UDD PMI 2014. Malang. Retrieved January 8, 2018, from https://bimaariotejo.wordpress.com/category/darah/

Budiningsih, Asri. 2010. Gambaran Faktor-Faktor Yang Memengaruhi Motivasi Pendonor Sukarela Untuk Mendonorkan Darah Di Utd-Pmi Kota Medan Tahun 2010. Retrieved January 8, 2018, from http://repository.usu.ac.id/bitstream/handle/123456789/33673/Chapter%20II.pdf?sequence=4

Dahlan, Muhammad Sopiyudin. 2011. Statistik untuk Kedokteran dan Kesehatan: Deskriptif, Bivariat, dan Multivariat, Dilengkapi Aplikasi dengan Menggunakan SPSS Edisi 5. Jakarta: Salemba Medika.

Departemen Kesehatan RI. 2008. Pedoman Pembinaan dan Pengawasan Pelayanan Darah oleh Dinas Kesehatan. Jakarta: Lampiran Departemen Kesehatan RI. Retrieved January 7, 2018 from http://perpustakaan.depkes.go.id:8180/bitstream//123456789/824/4/BK2008-G16.pdf

Faruq, Zulfikar Husni. 2015. Modul 3: Serologi Golongan Darah. Banten: Poltekkes Jakarta 3.

Kiswari, Rukman. 2014. Hematologi & TransfusiI. Jakarta: Erlangga.

Kitchen, Gareth. 2008. Immunology and haematology. China: Elsevier.

Kosasih, E.N., A.S. Kosasoh. 2008. Tafsiran Hasil Pemeriksaan Laboratorium Klinik. Tangerang: KARISMA Publishing Group.

Kresno, Siti Boedina. 1988. Pengantar Hematologi dan Imunohematologi. Jakarta: Gaya Baru.

Mutiawati, Vivi Keumala. 2013. Perbedaan Derajat Aglutinasi Pemeriksaan Golongan Darah Antara Eritrosit Tanpa Pencucian dengan Eritrosit Tanpa Pencucian Pada Penderita Talasemia. Retrieved November 19, 2017, from http://download.portalgaruda.org/article.php?article=428872&val=3947&title=PERBEDAAN%20DERAJAT%20AGLUTINASI%20PEMERIKSAAN%20GOLONGAN%20DARAH%20ANTARA%20ERITROSIT%20TANPA%20PENCUCIAN%20DENGAN%20PENCUCIAN%20PADA%20PENDERITA%20TALASEMIA

Penn Vet Research. 2016. Penn Vet Research Shows Success for New Testing Methods for Blood Compatibility, Typing. Retrieved January 8, 2018, from http://www.vet.upenn.edu/about/press-room/publications/new-bolton-post/new-bolton-post-summer-2016/penn-vet-research-shows-success-for-new-testing-methods-for-blood-compatibility-typing

Peraturan Menteri Kesehatan Republik Indonesia Nomor 83. 2014. Unit Transfusi Darah, Bank Darah Rumah Sakit, dan Jejaring Pelayanan Transfusi Darah. Retrieved December 17, 2017

Peraturan Pemerintah Republik Indonesia Nomor 7. 2011. Pelayanan Darah. Retrieved January 4, 2018

Samudra, Riska Indriati. 2017. Perbedaan Derajat Aglutinasi Berdasarkan Lama Penyimpanan Suspensi Sel Pada Pemeriksaan Golongan Darah Metode Tabung. Unpublished undergraduate diploma, Poltekkes Kemenkes Semarang, Semarang.

Siswanto, Susila, & Suyanto. 2016. Metodologi Penelitian Kesehatan DAN Kedokteran. Yogyakarta: Bursa Ilmu.

Soemantri, Ag, Tatty Ermin Setiati. 2009. Kegawatan Hematologi. Semarang: Pelita Insani.

Yuni, Natalia Erlina. 2015. Kelainan Darah. Yogyakarta: Nuha Medika.

WHO. 2002. Model Standard Operating Procedures for Blood Transfusion service. New Delhi: WHO. Retrieved January 4, 2018, from http://apps.searo.who.int/PDS_DOCS/B0235.pdf?ua=1

WHO. 2013. Standard Operating Procedures for Blood Transfusion. Bangladesh: WHO. Retrieved January 4, 2018, from http://www.who.int/bloodsafety/transfusion_services/sop-bts bangladesh.pdf




DOI: https://doi.org/10.31983/jlm.v1i1.5181

Article Metrics

Abstract view : 597
Download PDF (Bahasa Indonesia) : 15711

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Nur Muhjah Khodijjah

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

--

OFFICE INFORMATION :

Jurusan Analis Kesehatan - Politeknik Kesehatan Kemenkes Semarang, http://analis.poltekkes-smg.ac.id Jl. Wolter Monginsidi No. 115 Pedurungan Tengah, Semarang, Jawa Tengah, Indonesia ; Telp./Fax: (024)6723014 Email: jlm@poltekkes-smg.ac.id

Statcounter  Lisensi Creative Commons Jaringan Laboratorium Medis disseminated below Creative Commons Attribution-ShareAlike 4.0 International License.

Our Related Accounts :

 

  
MoU PATELKIISSN LIPIGoogle ScholarGarudaStat CounterDOI Crossref  

 

Jaringan Laboratorium Medis © 2019