Reviewers
Editorial Board
Copyright & Licensing
Screening Plagiarism
Online Submission
Register
Author Guidelines
Manuscript Template
The Effectiveness of Ylang Ylang Oil (Cananga Odorata) as a Deparaffinizing Agent in Hematoxylin-Eosin Staining
Abstract
Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses a xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylang oil, which contains β-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimed to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) ≥ 15, resulting in a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p<0.05). The Kruskal-Wallis test was then used.
Keywords
Full Text:
PDFReferences
Andila, P. S., Wibawa, I. P. A., Warseno, T., Li’aini, A. S., Tirta, I. G., Bangun, T. M. (2020). Seri Koleksi Kebun Raya Eka Karya Bali: Tanaman Berpotensi Penghasil Minyak Atsiri. Jakarta: LIPI Press. DOI: https://doi.org/10.14203/press.311
Anggia, M., Mutiar, S., Arziah, D. (2018). Teknologi Ekstraksi Bunga Kenanga (Cananga Odorata L.) dan Sereh Wangi (Cymbopogon Nardus L.) Sebagai Aroma Terapi Sabun Cair. Jurnal Daur Lingkungan, 1(1): 5-9. DOI: http://dx.doi.org/10.33087/daurling.v1i1.2
Badjuri, F. Z., Durachim, A., Wiryanti, W., Riyani, A., Dani, M. (2023). Pengaruh Variasi Suhu Dan Waktu Virgin Coconut Oil Pada Proses Deparafinisasi Pewarnaan Hematoksilin Eosin Terhadap Kualitas Preparat. Jurnal Kesehatan Siliwangi. 4(1):172-181. https://doi.org/10.34011/jks.v4i1.1473
Borgonetti, V., López, V., Galeotti, N. (2022). Ylang-ylang (Cananga odorata (Lam.) Hook. f. & Thomson) essential oil reduced neuropathic-pain and associated anxiety symptoms in mice. Journal of ethnopharmacology. 294: 115362. https://doi.org/10.1016/j.jep.2022.115362
Cahyana, G.H., Sukrisna, A., Mulyani, T. (2017). Hubungan Paparan Xylene Dan Methyl Hippuric Acid Pada Pekerja Informal Pengecatan Mobil Di Karasak, Bandung. Creative Research Journal. 1(01): 79- 94. https://doi.org/10.31219/osf.io/qa5bu
Chan, J.K. (2014). The wonderful colors of the hematoxylin-eosin stain in diagnostic surgical pathology. Int J Surg Pathol. 22(1):12-32. https://doi.10.1177/1066896913517939.
Damayanti, M., Ariyadi, T., Tyas, R. A. (2021). Proses Deparafinisasi sediaan jaringan ginjal dengan dan tanpa pemanasan menggunakan mineral oil pada pewarnaan hematoksili-eosin. Jurnal Kesehatan Rajawali 11(2): 1-6. DOI: https://doi.org/10.54350/jkr.v11i2.104
Dhifi, W., Bellili, S., Jazi, S., Bahloul, N., Mnif, W. (2016). Essential Oils' Chemical Characterization and Investigation of Some Biological Activities: A Critical Review. Medicines (Basel, Switzerland). 3(4) 25. https://doi.org/10.3390/medicines3040025
Hernandes, E. P., Schoffen, R. P. Conte, H., (2017). Xylene: Features, Risks and Management of Waste. Brazilian Journal of Surgery and Clinical Research, 17(2): 68-73. http://www.mastereditora.com.br/bjscr
Kandyala, R., Raghavendra, S. P., Rajasekharan, S. T. (2010). Xylene: An overview of its health hazards and preventive measures. Journal of oral and maxillofacial pathology: JOMFP, 14(1), 1–5. https://doi.org/10.4103/0973-029X.64299
Kristian, E. (2018). Potensi Minyak Gandapura sebagai Pengganti Xilol dalam Pembuatan Sediaan Mikroskopis Otak Mencit. Prosiding Pertemuan Ilmiah Nasional Penelitian & Pengabdian Masyarakat Dies Natalis ke-16 STIKES Jenderal Achmad Yani Cimahi Pinlitamas. 11(1): 638-644. https://repository2.stikesayani.ac.id/
Marinho, P.F., Hanscheid, T. (2023). A simple heat-based alternative method for deparaffinization of histological sections significantly improves acid-fast staining results for Mycobacteria in tissue. MethodsX. 12;10:102079. https://doi.10.1016/j.mex.2023.102079.
Mayangsari, M. A. Nuroini, F., Ariyadi, T. (2019). Perbedaan Kualitas Preparat Ginjal Marmut pada Proses Deparafinasi Menggunakan Xylol dan Minyak Zaitun pada Pewarnaan HE. Jurnal Nasional Unimus, 190- 193. http://prosiding.unimus.ac.id
Nuroini, F. Erwin, Y. Ariyadi, T. (2019). Proses Deparafinasi Sediaan Jaringan Ginjal Dengan Dan Tanpa Pemanasan Menggunakan Mineral Oil Pada Pewarnaan Hematoksilin-Eosin. Prosiding Mahasiswa Seminar Nasional Unimus. 2(2019):185-189 http://prosiding.unimus.ac.id
Patandung, P. (2019). Pengaruh Suhu Parafin Cair Terhadap Waktu Penyimpanan Rimpang Jahepengaruh Suhu Parafin Cair Terhadap Waktu Penyimpanan Rimpang Jahe. Jurnal Penelitian Teknologi Industri. 10(2);45. https://doi.10.33749/jpti.v10i2.4650
Pratiwi, E. N., Armalina, D. (2021). Mikroskopis Preparat Mus Musculus Jaringan Ginjal Yang Dideparafinisasi Dengan Minyak Zaitun Pada Pengecatan Hematoxylin Eosin (HE). Jurnal Laboratorium Medis. 3(1): 61-66. https://doi.org/10.31983/jlm.v3i1.8005
Prema, V., Prasad, H., Srichinthu, K. K., Kumar, S. S., Rajkumar, K., & Marudhamani, C. (2020). Biofriendly Substitutes for Xylene in Deparaffinization. Journal of pharmacy & bioallied sciences. 12(Suppl 1), S623–S630. https://doi.org/10.4103/jpbs.JPBS_164_20
Pujiarti, R., Widowati, T. B., Kasmudjo., Sunarta, S. (2015). Kualitas, Komposisi Kimia, dan Aktivitas Antioksidan Minyak Kenanga. Jurnal Ilmu Kehutanan 9(1): 3-11. https://doi.org/10.22146/jik.10179
Rina, S. (2013). Petunjuk Praktikum Mikroteknik. Bagian Histologi dan Biologi Sel. Yogyakarta: FK UGM. https://jurnal.ugm.ac.id/jpki/article/download/25322/16224
Sari, Y. E. S., Hariyanto. (2019). Rendaman Kuncup Daun Jati (Tectona grandis) sebagai Alternative Pewarna Eosin pada Proses Histoteknik. Prosiding Senakes 1.0. STIKES Rumah Sakit Anwar Medika: 76-80. https://repository.um-surabaya.ac.id/
Swamy, S. R., Nandan, S. R., Kulkarni, P. G., Rao, T. M., Palakurthy, P. (2015). Bio-Friendly Alternatives for Xylene - Carrot oil, Olive oil, Pine oil, Rose oil. Journal of clinical and diagnostic research: JCDR. 9(11). ZC16–ZC18. https://doi.org/10.7860/JCDR/2015/16384.6731
Tan, L. T., Lee, L. H., Yin, W. F., Chan, C. K., Abdul Kadir, H., Chan, K. G., Goh, B. H. (2015). Traditional Uses, Phytochemistry, and Bioactivities of Cananga odorata (Ylang-Ylang). Evidence-based complementary and alternative medicine: eCAM. 2015:896314. https://doi.org/10.1155/2015/896314
Udonkang, M., Eluwa, M., Ekanem, A., Sharma, T. B., Asuquo, O. R., Akpantah, A. O. (2014). Bleached palm oil as substitute for xylene in histology. J Pharm Clin Res. 8:8-17. www.arpapress.com/Volumes/JPCS/Vol8/JPCS_8_02.pdf
DOI: https://doi.org/10.31983/jlm.v6i1.10824
Article Metrics
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Jaringan Laboratorium Medis
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
--
OFFICE INFORMATION :
Jurusan Analis Kesehatan - Kemenkes Poltekkes Semarang, https://analis.poltekkes-smg.ac.id Jl. Wolter Monginsidi No. 115 Pedurungan Tengah, Semarang, Jawa Tengah, Indonesia ; Telp./Fax: (024)6723014 Email: jlm@poltekkes-smg.ac.id
Jaringan Laboratorium Medis disseminated below Creative Commons Attribution-ShareAlike 4.0 International License.
Our Related Accounts :
 |  |  |  |  |  | ||
| MoU PATELKI | ISSN BRIN | Google Scholar | Garuda | Stat Counter | DOI Crossref |
| Jaringan Laboratorium Medis © 2019 |