Antibacterial Effect of Mint Leaf and Basil Leaf Extract addition to Pineapple Hump Extracts Against Streptococcus Mutans Bacteria

Naning Nur Handayatun, David Rudi, Haflin Haflin, Qurati Ayun


The use of herbal ingredients for dentistry is preferable to the use of chemicals. Pineapple is one of the fruits that can be used in dentistry. Pineapple hump is often not consumed even though it still contains many bromelains. This study aimed to see the antibacterial effect of adding mint and basil leaf extract into pineapple hump extract. This type of research is a pure experimental study with a control group design and partly water. This study's experimental group or independent variables were pineapple hump extract, mint leaf extract, and basil leaf extract. The control group was Chlorhexidine and water. The variable is Streptococcus mutans. The extract was made by maceration technique, and the examination of the inhibition of bacteria was carried out using an oxoid blank disc. There was a difference in inhibition against Streptococcus mutans between the experimental and control groups p<0.05, but there was no difference between the pineapple hump extract with the addition of min leaf extract and the pineapple weevil extract with the addition of basil leaves. Adding mint and basil leaf extract to pineapple hump extract can increase its antibacterial power against Streptococcus mutans.


antibacterial; basil leaf; mint leaf; pineapple hump; Streptococcus mutans

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J. A. Lemos et al., “The biology of Streptococcus mutans,” Microbiol Spectr, vol. 7, no. 1, 2019.

R. A. Praveen, NC Madan M, Chaurasia VR, “Invitro evaluation of antibacterial Efficacy of pineapple exctract (Bromelin) on periodontal pathogen,” Journal of InternationalOral Health, vol. 6, no. 5, pp. 96–98, 2014.

M. F. Hossain, S. Akhtar, and M. Anwar, “Nutritional value and medicinal benefits of pineapple,” International Journal of Nutrition and Food Sciences, vol. 4, no. 1, pp. 84–88, 2015.

N. C. Praveen, A. Rajesh, M. Madan, V. R. Chaurasia, N. v Hiremath, and A. M. Sharma, “In vitro evaluation of antibacterial efficacy of pineapple extract (bromelain) on periodontal pathogens,” J Int Oral Health, vol. 6, no. 5, 2014.

N. C. Praveen, A. Rajesh, M. Madan, V. R. Chaurasia, N. v Hiremath, and A. M. Sharma, “In vitro evaluation of antibacterial efficacy of pineapple extract (bromelain) on periodontal pathogens,” J Int Oral Health, vol. 6, no. 5, 2014.

A. Y. Bahtiyar, O. Efriyadi, and E. Fitriah, “Efektivitas Kandungan Anti-Bakteri Buah Nanas (Ananas comosus L. Merr) dalam Menghambat Pertumbuhan Bakteri Streptococcus mutans,” Seminar Nasional Sains dan Entrepreneurship IV Tahun 2017, 2017.

G. I. A. Ali A Milala MA, “Anti Micobial Effect of crude broelin extracted from pineapple fruits ( Ananas comosus (Linn,) Merr).,” Advance in Biochemistry, vol. 3, no. 1, p. 27, 2015.

2016. Manzoor Z, Nawaz A, Mukhtar H, Haq I, “Bromelain: Methods of Extraction, Purification andTherapeutic Applications. An,” International Journal of Brazilian Archives OfBiology And Technology, vol. 59, no. e16150010, 2016.

N. N. Handayatun, D. Rudi, and K. T. Fitria, “Pineapple Fruit and Stem Chewing PreventsDental Plaque pH Drop after Meals,” in Health, Social Sciences And Technology, May 2021, pp. 51–54.

W. Widyastuti, H. R. Fantari, V. R. Putri, and I. Pertiwi, “Formulasi pasta gigi ekstrak kulit jeruk (Citrus sp.) dan daun mint (Mentha piperita L.) serta aktivitas terhadap bakteri Streptococcus mutans,” Jurnal Pharmascience, vol. 6, no. 2, pp. 111–119, 2019.

D. A. Dhale, A. R. Birari, and G. S. Dhulgande, “Preliminary Screening of Antibacterial and Phytochemical Studies of Ocimum americanum Linn,” Journal of Ecobiotechnology, vol. 2, no. 8, 2010.

J. Supranto, Statistik Teori dan Aplikasi, 1st ed. Jakarta: Erlangga, 2000.

S. Kabir, J. S. Mehbish, and M. Mahboob, “Apple, guava and pineapple fruit extracts as antimicrobial agents against pathogenic bacteria,” Am J Microbiol Res, vol. 5, no. 5, pp. 101–106, 2017.

Z. Golestannejad et al., “Comparison of antibacterial activity of essential oils of Foeniculum vulgare Mill, Mentha arvensis and Mentha piperita against Streptococcus mutans,” Advanced Herbal Medicine, vol. 3, no. 1, 2017.

B. Giridharan, C. Amutha, N. Siddhan, and A. Ganeshkumar, “Antibacterial activity of Mentha piperita L. (peppermint) from leaf extracts - A medicinal plant,” Acta Agric Slov, vol. 89, no. 1, pp. 73–79, 2007.


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