The Effect Of Isotonic Solution Immersion On Tooth Enamel Hardness After Topical Application Of CPP-ACPF And fTCP

Sri Wahyuni, Rini Bikarindrasari, Mutiara Nur Fauziah


Introduction:  The  addition  of  topical  CPP-ACPF  and  fTCP  on  tooth  enamel  can improve enamel hardness due to remineralization process. Demineralization can occur during  acid  environment  in  the oral  cavity  and  enamel  hardness  can  decrease. Objective:  To determine  the effect of isotonic solution immersion  and compare tooth enamel hardness after CPP-ACPF and fTCP application. Methods: Thirty six crowns of  maxillary first premolars were divided into three groups, each consisted of 12 tooth crowns.  The  baseline  hardness  of  each  sample  (pre-test)  was  tested  using  Vicker’s Hardness Tester Digital. Afterwards,  CPP-ACPF  was applied to group A, fTCP was applied to group B, and artificial saliva to group C (control) for 24 hours, then the hardness was re-assessed  (post-test 1). Immersion in isotonic solution was conducted for  24  hours  to  simulate  demineralization  process,  then  enamel hardness  was  re- assessed  (post-test  2). Results:  Paired  t-test  on post-test  1 revealed  that all groups showed  significant  increase  and  post-test  2  showed  significant  enamel   hardness decrease.  The results  of  one-way  ANOVA  on  pre-test  showed  that  there  was  no significant difference, while post test 1 and post-test 2 revealed significant difference. Post-Hoc Bonferroni test showed that there was no statistically significant difference of enamel hardness between groups. Conclusion: There was an effect of isotonic solution immersion on tooth enamel hardness that were given topical CPP-ACPF and fTCP.


CPP-ACPF, demineralization, fTCP, enamel hardness, isotonic solution

Full Text:



John MK, Babu A, Gopinathan AS. Incipient caries: an early intervention approach. Int J Community Med Public Health 2015;2:10-4.

Andrini M, Titien J, Rantinah SB. Pengaruh aplikasi topical Casein Phospopeptide-Amorphous Calcium Phosphate (CPP-ACP) terhadap pertumbuhan streptococcus alpha dan akumulasi plak gigi. J Ked. Gigi.2013;4(4):267-273

Rahayu YC. Peran agen remineralisasi pada karies dini. Stomatogantic JKG Unej 2013;10:25-30.

Gultom E, Dyah R. Konsep dasar pelayanan kesehatan gigi dan mulut I.Jakarta: Kemenkes RI. 2017.p.19.

AlAmoudi SA, Rani SC, Alomari M. The effect of addition of Tri Calcium Phosphate to 5% Sodium fluoride Varnishes on the microhardness of Enamel of Primary teeth. Hindawi Pub Corp. International Journal of Dentistry. 2013:1-5.

Coombes JS. Sport Drinks and Dental. Am J Dent. 2015;18:101-104

Rachma PK. Kebiasaan minum, kebutuhan cairan, dan kecenderungan dehidrasi siswi sekolah dasar. Bogor: Departemen Gizi Masyarakat. Fakultas Ekologi Manusia IPB; 2009:49-50

Chole D, Jadhav Y, Kundoor S, Bakle S, Devagirkav A, Deshpande R. Remineralizing Agents: minimal invasive therapy A Review. IOSR Journal. 2016;15(2):64-68

Fejerskov O, Kidd E. Dental Caries: The Disease and Its Clinical Management. 2nd ed. Tunbridge Wells, UK: Blackwell Munksgaard Ltd; 2008. p.20-4, 202-27, 241-2, 318

Roberson TM, Heymann H, ES. Studervant’s Art and Science of Operative Dentistry. 6th ed. St. Louis: Mosby; 2012. p. 41-3

Margeas R. Remineralization with a unique delivery system. Insode Dentistry. 2014;4(2):86

Coombes JS. Sport Drinks and Dental. Am J Dent. 2015;18:101-104

Karlinsey RL, Pfarrer AM. Fluoride Plus Functionalized ß-TCP: A Promising Combination for Robust Remineralization. Adv Dent Res. 2012; 24(2): 48-52

Bayrak S, Tuloglu N, Bicer H, Tunc E. Sen. Effect of fluoride varnish containing CPP-ACP on preventing enamel erosion. Hindawi. 2017: 20-25

Dawes C. What is the critical pH and why does a tooth dissolve in acid. J Can dental Association; 2003; 69: 722-724


Article Metrics

Abstract view : 186
Download PDF : 220


  • There are currently no refbacks.

Jurnal Kesehatan Gigi (p-ISSN: 2407-0866 e-ISSN: 2621-3664), is published by Jurusan Kesehatan Gigi, Politeknik Kesehatan Kemenkes Semarang, Jl. Tirto Agung, Pedalangan, Banyumanik, Semarang, Jawa Tengah, Indonesia, 50268 Telp./Fax: (024) 7471276.

Find us on : Instagram, Facebook

Abstracted/Indexed by:


View My Stats