Examining the Impact of Porphyromonas gingivalis Infection on Ovarian Health: A Reduction in Primordial Follicles in a Female Periodontitis Model

Zahara Meilawaty, Argha Sirna Galau, Sabania Dinar Permatasari, Sari Setyaningsih, Pudji Astuti, Ernie Maduratna Setiawatie, Hendy Hendarto, Agustin Wulan Suci Dharmayanti

Abstract


Periodontitis is a chronic inflammation of the periodontal tissues caused by periodontal pathogens, such as Porphyromonas gingivalis (P. gingivalis). P. gingivalis possesses several virulence factors that enable it to invade and spread through the bloodstream to other organs, including the ovaries. It triggers inflammation, which may impact the number of primordial follicles. The aim of this study was to assess the effects of P. gingivalis infection on the number of primordial follicles in the ovaries. This study was a laboratory experiment with a post-test control group design. The rats were divided into control and periodontitis groups. The periodontitis group consisted of rats induced with P. gingivalis in their sulcus gingiva of the first mandible molar every three days for 19 days. Therefore, control groups were without P. gingivalis induction. Seven days after the last induction of P. gingivalis, the rats were euthanized, and their ovarian tissues were collected for histological preparation. The primordial follicles were counted under 400x magnification. The results indicated that the treatment group had a significantly lower number of primordial follicles (2.2 ± 2.61) compared to the control group (12.4 ± 2.41). The study concludes that P. gingivalis infection led to a reduction in the number of ovarian primordial follicles in periodontitis models.

Keywords


Primordial follicle; Ovaries; Periodontitis; P. gingivalis

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References


A. W. S. Dharmayanti, and B. Kusumawardani, “Deoxypyridinoline and Mineral Levels in Gingival Crevicular Fluid as Disorder Indicators of Menopausal Women with Periodontal Disease,” Dent. J. (Majalah Kedokt. Gigi), vol. 50, no. 32, 2017, pp. 131 137, doi: 10.20473/j.djmkg.v50.i3.p131-137.

I. K. E. Wijaksana, “Periodontal Chart dan Periodontal Risk Assessment sebagai Bahan Evaluasi dan Edukasi Pasien dengan Penyakit Periodontal,” J. Kesehat. Gigi, vol. 6, no. 1,2019, p. 19, doi: 10.31983/jkg.v6i1.4032.

G. G. Nascimento, S. Alves-Costa, and M. Romandini, “Burden of Severe Periodontitis and Edentulism in 2021, with projections up to 2050: The Global Burden of Disease 2021 study,” J. Periodontal Res., vol. 59, no. August, 2024, 10.1111/jre.13337. p

M. X. Chen, Y. J. Zhong, Q. Q. Dong, H. M. Wong, and Y. F. Wen, “Global, Regional, and National Burden 0f Severe Periodontitis, 1990–2019: An analysis of the Global Burden of Disease Study 2019,” J. Clin. Periodontol., vol. 48, no. 9, 2021, pp. 1165–1188, doi: 10.1111/jcpe.13506.

E. Mardiati, A. Subekti, E. N. Donasari, and R. Oktaviani, “Perbedaan Ph Saliva terhadap Pemakai Kontrasepsi Hormonal Pil dan Suntik pada Wanita Usia 20-40 Tahun di Kelurahan Gedawang Kota Semarang,” J. Kesehat. Gigi, vol. 7, no. 1, 2020, pp. 13–20, doi: 10.31983/jkg.v7i1.5519.

E. A. Ekoningtyas, I. H. Y. Siregar, and S. J. Sukendro, “the Effect of Giving Education about Rheological Saliva To Potential Quality of Mouth Saliva in Menopause Women,” J. Kesehat. Gigi, vol. 5, no. 2, 2018, p. 52, doi: 10.31983/jkg.v5i2.3862.

T. R. Septiwidyati and E. W. Bachtiar, “The role of Porphyromonas gingivalis Proteinases in Periodontitis,” dentika Dent. J., vol. 23, no. 1, 2020, pp. 6–12.

Y. Martayuda, C. Kurnia, and V. K. Sugiaman, “Antibacterial Activity of Andaliman Ethanol Extract (Zantoxylum acanthopodium Dc.) Against Porphyromonas gingivalis Bacteria,” J. Kesehat. Gigi, vol. 10, no. 2, 2023, pp. 10.31983/jkg.v10i2.10866. 108–115, doi:

C. A. de Jongh, T. J. de Vries, F. J. Bikker, S. Gibbs, and B. P. Krom, “Mechanisms of Porphyromonas gingivalis to Translocate over The Oral Mucosa and Other Tissue Barriers,” J. Oral Microbiol., vol. 15, no. 1, 2023, doi: 10.1080/20002297.2023.2205291.

A. W. S. Dharmayanti, S. Puspita, D. W. A. Fatmawati, and T. Ermawati, “Systematic Review: Porphyromonas gingivalis Gingipain Plays a Vital Role in Organ Dysfunction in Systemic Diseases,” Proceeding Improv. Qual. Dent., vol. 1, no. 1, 2024, pp. 6–21, doi: 10.18196/imunity.v1i1.2.

A. Nirola, P. Batra, and J. Kaur, “Ascendancy of Sex Hormones on Periodontium During Reproductive Life Cycle of Women,” J. Int. Clin. Dent. Res. Organ., vol. 10, no. 1, 2018, p. 3, doi: 10.4103/jicdro.jicdro_29_17.

J. Kotz, N. Chegini, K. T. Shiverick, and R. J. Lamont, “Localization of P. gingivalis in Preterm Delivery Placenta,” J. Dent. Res., vol. 88, no. 6, 2009, pp. 575–578, doi: 10.1177/0022034509338032. [13] L. A. Gómez et al., “Porphyromonas gingivalis Placental Atopobiosis and Inflammatory Responses in Women With Adverse Pregnancy Outcomes,” Front. Microbiol., vol. 11, no. December, 2020, doi: 10.3389/fmicb.2020.591626.

J. J. Bromfield and I. M. Sheldon, “Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1,” Biol. Reprod., vol. 88, no. 4, 2013,pp. 1–9, doi: 10.1095/biolreprod.112.106914.

N. Yamamoto et al., “Lipopolysaccharide (LPS) Suppresses Follicle Development Marker Expression and Enhances Cytokine Expressions, Which Results in Fail to Granulosa Cell Proliferation in Developing Follicle in Cows,” Reprod. Biol., vol. 23, no. 1, 2023, p. 100710, 10.1016/j.repbio.2022.100710. doi:

Rongkun Liu, T. Desta, M. Raptis, R. P. Darveau, and D. T. Graves, “Porphyromonas gingivalis and E. coli Lipopolysaccharide Exhibit Different Systemic but Similar Local Induction of Inflammatory Markers,” J Periodontol., vol. 79, no. 7, 2008, pp. 1241 1247, doi: 10.1902/jop.2008.070575.

A. W. S. Dharmayanti, Z. Meilawaty, T. Ermawati, A. M. Putradjaka, and Z. Hamzah, “Effect of Ovarian Dysfunction Induced Ovariectomy and Porphyromonas gingivalis Induction To Risk of Metabolic Syndrome: In Vivo Study,” Brazilian Dent. Sci., vol. 27, no. 1, 2024, pp. 10.4322/bds.2024.e3880. 1–12, doi:

A. W. S. Dharmayanti et al., “Superoxide Dismutase Expression in the Ovaries of Periodontitis Animal Models Induced by Porphyromonas gingivalis and Treated with Cassava Leaves Extract,” Ann. Dent., vol. 28, 2021, pp. 10.22452/adum.vol28no7. 40–46, doi:

E. A. Fuller et al., “Neonatal Immune Activation Depletes The Ovarian Follicle Reserve and Alters Ovarian Acute Inflammatory Mediators in Neonatal Rats,” Biol. Reprod., vol. 97, no. 5, 2017, pp. 719 730, doi: 10.1093/biolre/iox123.




DOI: https://doi.org/10.31983/jkg.v12i1.12547

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