Jaringan Laboratorium Medis

Vol. 8 No. 2 (2026): November 2026
Original Articles

Cytotoxicity of Papaya Flower Extract (Carica Papaya L) against T47D Breast Cancer Cells

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  • Rachmad Bayu Kuncara,
  • Rizqi Mukramiin Marpaung,
  • Teguh Budiharjo,
  • Rodhi Hartono
Rachmad Bayu Kuncara
Poltekkes Kemenkes Semarang
Rizqi Mukramiin Marpaung
Poltekkes Kemenkes Semarang
Teguh Budiharjo
Poltekkes Kemenkes Semarang
Rodhi Hartono
Poltekkes Kemenkes Semarang
DOI: https://doi.org/10.31983/jlm.v8i2.15430

Published 2026-10-01

Keywords

  • Breast Cancer ,
  • Carica Papaya L flower extract ,
  • T47D,
  • MTT Assay

How to Cite

Kuncara, R. B., Rizqi Mukramiin Marpaung, Teguh Budiharjo, & Rodhi Hartono. (2026). Cytotoxicity of Papaya Flower Extract (Carica Papaya L) against T47D Breast Cancer Cells. Jaringan Laboratorium Medis, 8(2), 147–153. https://doi.org/10.31983/jlm.v8i2.15430

Copyright (c) 2026 Jaringan Laboratorium Medis

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Abstract

Breast cancer is the type of cancer with the highest prevalence in Indonesia that affects women. Carica papaya L is classified as a plant that has various activities as antioxidants and anti-cancer due to the content of phytochemicals, including flavonoids, saponins, and tannins that can induce apoptosis of cancer cells. This study aims to determine the cytotoxicity of papaya flower extract (Carica papaya L) against T47D breast cancer cells based on IC50 value. This research is a pure experiment. T47D breast cancer cell culture group was given papaya flower extract (Carica papaya L.) with a concentration series, namely 100, 200, 300, 400 and 500 μg/ml. After that, the cytotoxic test of MTT assay method was conducted. The results indicated that an increase in treatment concentration had an impact on decreasing cell viability. The final result obtained IC50 value using Graphad Prism software obtained IC50 value on papaya flower extract of 115.6 µg/ml which shows moderate cytotoxic activity against T47D breast cancer cells. Based on this study, it can be concluded that papaya flower extract (Carica papaya L) affects the viability of T47D breast cancer cells with an IC₅₀ value of 115.6 µg/ml.

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