Comparison of Various Sporary Methods on Bacillus Cereus

Widodo Widodo, Devi Etivia Purlinda, Ahmad Riadi

Abstract


Endospores are formed by members of several genera of gram-positive bacteria, such as Bacillus and Clostridium. The spores are very resistant structurally; withstand the temperature of boiling water for two hours or more. They contain very little water and show very few chemical reactions. The favorable external environment will cause the spores to break down and the vegetative cells to appear to grow and reproduce. Identification of endospore-forming pathogens is important in food and medical microbiology. However, the spores contain multiple protective layers, which cannot be easily penetrated by simple staining or Gram staining techniques. Therefore, it is necessary to apply heat to help penetrate the color, with various methods of Gram stain, fluorescent spore painting, Schaeffer and Fulton spore painting and Client's spore painting method. The results of the fluorescent painting method have better results but require much more expensive equipment using a fluorescent microscope considering the facilities that must be provided for the Schaeffer and Fulton painting method as an alternative if they do not have these facilities.


Keywords


Bacillus Cereus ; Spores ; Painting Method

References


Ehling-Schulz, M., Lereclus, D., and Koehler, T. M. (2019). The Bacillus cereus group: Bacillus species with pathogenic potential. Microbiology spectrum, 7(3), 7-3.

Tuipulotu, D. E., Mathur, A., Ngo, C., & Man, S. M. (2021). Bacillus cereus: epidemiology, virulence factors, and host–pathogen interactions. Trends in Microbiology, 29(5), 458-471.

Ehling-Schulz, M., Frenzel, E., and Gohar, M. (2015). Food–bacteria interplay: pathometabolism of emetic Bacillus cereus. Frontiers in microbiology, 6, 704.

Oktari, A., Supriatin, Y., Kamal, M., & Syafrullah, H. (2017, February). The bacterial endospore stain on Schaeffer Fulton using variation of methylene blue solution. In Journal of Physics: Conference Series (Vol. 812, No. 1, p. 012066). IOP Publishing.

Smith, A. C., & Hussey, M. A. (2005). Gram stain protocols. American Society for Microbiology, 1, 14.

Thairu, Y., Nasir, I. A., and Usman, Y. (2014). Laboratory perspective of gram staining and its significance in investigations of infectious diseases. Sub-Saharan African Journal of Medicine, 1(4), 168.

Gurung, R., Shrestha, R., Poudyal, N., & Bhattacharya, S. K. (2018). Ziehl Neelsen vs. Auramine staining technique for detection of acid fast bacilli. Journal of BP Koirala Institute of Health Sciences, 1(1), 59-66.

Schichnes, D., Nemson, J. A., and Ruzin, S. E. (2006). Fluorescent staining method for bacterial endospores. MICROSCOPE-LONDON THEN CHICAGO-, 54(2), 91.

Driks, A. (1999). Bacillus subtilis spore coat. Microbiology and Molecular Biology Reviews, 63(1), 1-20.

Oktari, A., Supriatin, Y., Kamal, M., and Syafrullah, H. (2017). The Bacterial Endospore Stain on Schaeffer Fulton using Variation of Methylene Blue Solution. In Journal of Physics: Conference Series (Vol. 812, No. 1, p. 012066). IOP Publishing.




DOI: https://doi.org/10.31983/jlm.v4i2.8493

Article Metrics

Abstract view : 126
Download PDF (Bahasa Indonesia) : 51

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Jaringan Laboratorium Medis

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

--

OFFICE INFORMATION :

Jurusan Analis Kesehatan - Politeknik Kesehatan Kemenkes Semarang, http://analis.poltekkes-smg.ac.id Jl. Wolter Monginsidi No. 115 Pedurungan Tengah, Semarang, Jawa Tengah, Indonesia ; Telp./Fax: (024)6723014 Email: jlm@poltekkes-smg.ac.id

Statcounter  Lisensi Creative Commons Jaringan Laboratorium Medis disseminated below Creative Commons Attribution-ShareAlike 4.0 International License.

Our Related Accounts :

 

  
MoU PATELKIISSN LIPIGoogle ScholarGarudaStat CounterDOI Crossref  

 

Jaringan Laboratorium Medis © 2019