Biochemistry Mycobacterium Tuberculosis Treated with Anti-Isoniazid Treatment

Elly Karlina, Widodo Widodo, Devi Etivia Purlinda

Abstract


Mycobacterium tuberculosis exposed to Isoniazid experienced drug resistance due to morphological and selection factors, with Isoniazid concentrations of 0.5 μg / ml with a contact time of 24 hours, 48 hours, 72 hours, 96 hours, the results obtained in testing for NaCl 5% Kruskal wallis obtained sig 0.005 results there is a treatment effect on growth While in the semi-quantitative catalase test, a sig value of 0.007 was obtained with p <0.01 so it can be concluded that there is a significant influence between the duration of isoniazid exposure. resistance occurs due to the selection of strains that are resistant to treatment during the therapy process at 96 hours exposure with a percentage of 2%. In the exposure process it can be concluded that it will change the resistance to 5% NaCl, changes in Catalase test results and also causes strain selection that is resistant to isoniazid 0.5 µg / ml.


Keywords


Mycobacterium tuberculosis ; Isoniazid ; Biochemistry

References


Asmar, S., Sassi, M., Phelippeau, M., and Drancourt, M. 2016. Inverse correlation between salt tolerance and host-adaptation in mycobacteria. BMC research notes, 9(1), 1-9. https://doi.org/10.1186/s13104-016-2054-y

Babady, N. E., and Wengenack, N. L. 2012. Clinical laboratory diagnostics for Mycobacterium tuberculosis, understanding tuberculosis—global experiences and innovative approaches to the diagnosis. ISBN, 978-953.

Boum, Y., Orikiriza, P., Rojas-Ponce, G., Riera-Montes, M., Atwine, D., Nansumba, M., Bazira, J., Tuyakira,E., De Beaudrap, P., Bonnet, M., and Page, A. L. 2013. Use of colorimetric culture methods for detection of Mycobacterium tuberculosis complex isolates from sputum samples in resource-limited settings. Journal of Clinical Microbiology 51(7): 2273-2279. DOI: 10.1128/JCM.00749-13

Conville, P. S., and Witebsky, F.G. 1998. Variables affecting results of sodium chloride tolerance test for identification of rapidly growing mycobacteria. Journal of Clinical Microbiology 36.6 : 1555-1559. DOI: 10.1128/JCM.36.6.1555-1559.1998

Crofton, John, and D. A. Mitchison. 1948. Streptomycin resistence in pulmonary tuberculosis. British Medical Journal 2.4588 : 1009. doi: 10.1136/bmj.2.4588.1009

Kochi, A., Vareldzis, B., and Styblo, K. 1993. Multidrug-resisten tuberculosis and its control. Research in microbiology, 144(2): 104-110. https://doi.org/10.1016/0923-2508(93)90023-U

Larrouy-Maumus, G., Marino, L. B., Madduri, A. V., Ragan, T. J., Hunt, D. M., Bassano, L., Gutierrez, M.G., Moody, D.B., Pavan, F. R., and de Carvalho, L. P. S. 2016. Cell-Envelope Remodeling as a Determinant of Phenotypic Antibacterial Tolerance in Mycobacterium tuberculosis. ACS Infectious Diseases 2(5): 352-360. doi.org/10.1021/acsinfecdis.5b00148

Ramaswamy, S. V., Reich, R., Dou, S. J., Jasperse, L., Pan, X., Wanger, A., Quitugua, T., and Graviss,A, 2003. Single nucleotide polymorphisms in genes associated with Isoniazid resistence in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy, 47 : 1241-1250. doi.org/10.1021/acsinfecdis.5b00148

Widodo, W., Irianto, A., dan Pramono, H. 2017. Karakteristik Morfologi Mycobacterium tuberculosis yang Terpapar Obat Anti TB Isoniazid (INH) secara Morfologi. Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal, 33(3), 109-115. Doi 10.20884/1.mib.2016.33.3.316

Zhang, Y., Garbe, T., and Young, D. 1993. Transformation with Kat G restores Isoniazid‐sensitivity in Mycobacterium tuberculosis isolates resisten to a range of drug concentrations. Molecular Microbiology, 8(3): 521-524. doi.org/10.1111/j.1365-2958.1993.tb01596.x

Zhang, Y., Heym, B., Allen, B., Young, D., and Cole, S. 1992. The catalase—peroxidase gene and Isoniazid resistence of Mycobacterium tuberculosis, Nature, 358 (6387), 591 – 593. doi.org/10.1038/358591a0

Zhang, Y., and Yew, W. W. 2009. Mechanisms of drug resistence in Mycobacterium tuberculosis [State of the art series. Drug-resisten tuberculosis. Edited by CY. Chiang. Number 1 in the series]. The International Journal of Tuberculosis and Lung Disease, 13(11): 1320-1330.




DOI: https://doi.org/10.31983/jlm.v1i2.5871

Article Metrics

Abstract view : 512
Download PDF (Bahasa Indonesia) : 1192

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Jaringan Laboratorium Medis

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

--

OFFICE INFORMATION :

Jurusan Analis Kesehatan - Kemenkes Poltekkes Semarang, https://analis.poltekkes-smg.ac.id Jl. Wolter Monginsidi No. 115 Pedurungan Tengah, Semarang, Jawa Tengah, Indonesia ; Telp./Fax: (024)6723014 Email: jlm@poltekkes-smg.ac.id

Statcounter  Lisensi Creative Commons Jaringan Laboratorium Medis disseminated below Creative Commons Attribution-ShareAlike 4.0 International License.

Our Related Accounts :

 

  
MoU PATELKIISSN BRINGoogle ScholarGarudaStat CounterDOI Crossref  

 

Jaringan Laboratorium Medis © 2019