Degree of Agglutination in Tube Method Blood Group Examination Based on Day 0, 2, 4, 6, and 8

Karinta Syifa Zatalini, Rachmad Bayu Kuncara, Anung Sugihantono

Abstract


Blood group examination is an examination that aims to determine the type of blood group. Cell test is a blood group examination reagent used to detect antibodies in the serum being examined. The long shelf life of cell tests that can only last for two days is considered less effective for agencies with a high level of blood services. The purpose of this study was to describe the degree of agglutination in blood group examination with cell test A and B stored on the 0th, 2nd, 4th, 6th, and 8th days. This study is a descriptive study with a Quasi Experimental research design. Test cell A and test cell B were made from red blood cell specimens of 3 blood type A and 3 blood type B respectively. Test cells are stored in a refrigerator with a temperature of 2-6° C. Test cells were then examined on the 0th, 2nd, 4th, and 6th day of storage. Calculation of samples and repetitions using the Federer formula with the number of treatments in this study is 5 treatments. Based on the calculation, one sample of test cell A and test cell B was obtained with five repetitions of each examination. The results showed that on the 0th, 2nd, 4th, and 6th day of cell test storage, the results of blood type examination were obtained, namely the degree of agglutination 4+ with erythrocytes in the cell test clumping into one bond, cells forming large agglutination with clear supernatant. On the 8th day of storage, the result of agglutination degree is 3+ with erythrocytes in test cells not clumping perfectly, there are erythrocyte granules and cloudy supernatant. Based on the results of the study, it is concluded that test cell A and test cell B can be used optimally until day 6 storage.



Keywords


Agglutination Degree ; Shelf Life ; Test Cell ; Blood Type ; Serum Grouping

Full Text:

PDF

References


Afriansyah, F., Bastian, B., Sari, I., & Juraijin, D. (2021). Perbedaan Darah Segera Diperiksa, Dilakukan Penyimpanan Pada Suhu 20oC-25oC Dan 4oC-8oC Selama 6 Jam Terhadap Jumlah Eritrosit. Journal of Indonesian Medical Laboratory and Science (JoIMedLabS), 2(2), 108–114. DOI: https://doi.org/10.53699/joimedlabs.v2i2.51

Aldi, Y., Wahyuni, F. Sri., Dillasamola, D., Badriyya, E., & Srangenge, Y. (2023). Serologi Imunologi. Padang: Andalas University Press. http://repo.unand.ac.id/id/eprint/49703

Ammariah, H., Nurhidayanti, Bastian, dan Kartika, T. (2022). Perbedaan Hasil Derajat Aglutinasi Serum Grouping Tube Test Dengan Suspensi Reagen NaCl 0,9% Siap Pakai Dan Suspensi Reagen NaCl 0,9% Dari Garam Dapur. Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam 19(2), 208–14. DOI: https://doi.org/10.31851/sainmatika.v19i2.9500

Ananda, H. R., Hartati, D., & Juraijin, D. (2024). Perbedaan Derajat Aglutinasi Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Konsentrasi Suspensi Sel 5% Segera Periksan Dengan Lama Penyimpanan 5 Hari. JHAST (Journal Health Applied Science and Technology), 2(1), 6–13. DOI: https://doi.org/10.52523/jhast.v2i1.32

Arviananta, R., Syuhada, S., & Aditya, A. (2020). Perbedaan Jumlah Eritrosit Antara Darah Segar dan Darah Simpan di UTD RSAM Bandar Lampung. Jurnal Ilmiah Kesehatan Sandi Husada, 9(2), 686–694. https://doi.org/10.35816/jiskh.v12i2.388

Fauziyah, Z., Hayati, E., Nurhayati, B., & Marliana, N. (2019). Stabilitas Prc Dalam Larutan Alsever Buatan Terhadap Morfologi Eritrosit Dan Fragilitas Osmotik. Jurnal Riset Kesehatan Poltekkes Depkes Bandung, 11(1), 277–284. https://doi.org/10.34011/juriskesbdg.v11i1.779

Li, H. Y., dan Guo, K. (2022). Blood Group Testing. Frontiers in Medicine, (9), 1–11. doi: 10.3389/fmed.2022.827619

Li, X., Li, M., Wang, Y., Duan, S., Wang, H., Li, Y., Cai, Z., Wang, R., Gao, S., Qu, Y., Wang, T., Cheng, F., & Liu, T. (2023). The Development and Application Of A Novel Reagent For Fixing Red Blood Cells With Glutaraldehyde and Paraformaldehyde. Hematology (United Kingdom), 28(1). https://doi.org/10.1080/16078454.2023.2204612

Maharani, E. A., dan Noviar, G. (2018). Imunohematologi dan Bank Darah. Jakarta: Pusat Pendidikan Sumber Daya Manusia Kesehatan Kemenkes RI

Mulyantari, N. K. & Yasa, I. W. P. S. 2016. Laboratorium Pratransfusi. Denpasar: Udayana University Press

Naid, T., Arwie, D., & Mangerangi, F. (2012). Pengaruh Waktu Penyimpanan Terhadap Jumlah Eritrosit Darah Donor. Jurnal Ilmiah As-Syifaa, 4(1), 112–120. https://doi.org/10.33096/jifa.v4i1.149

Oktari, A., dan Silvia, N. D. (2016). Pemeriksaan Golongan Darah Sistem ABO Metode Slide Dengan Reagen Serum Golongan Darah A, B, O. Jurnal Teknologi Laboratorium 5(2), 49–54. http://www.teknolabjournal.com/index.php/Jtl/article/view/13/10

Ritchie, N. K., & Hawwa, S. A. (2021). Uji Stabilitas Ph dan Morfologi Sel Darah Merah Uji pada Larutan Alsever dan Nacl 0,9% di Unit Donor Darah Pusat PMI. Ensiklopedia of Journal, 03(5), 127–131. DOI: https://doi.org/10.33559/eoj.v4i3.311

Songjaroen, T., & Laiwattanapaisal, W. (2016). Simultaneous Forward And Reverse ABO Blood Group Typing Using A Paper-Based Device And Barcode-Like Interpratation. Analytica Chimica Acta, 921, 67–76. https://doi.org/10.1016/j.aca.2016.03.047




DOI: https://doi.org/10.31983/jlm.v6i2.11733

Article Metrics

Abstract view : 78
Download PDF : 19

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Jaringan Laboratorium Medis

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

--

OFFICE INFORMATION :

Jurusan Analis Kesehatan - Kemenkes Poltekkes Semarang, https://analis.poltekkes-smg.ac.id Jl. Wolter Monginsidi No. 115 Pedurungan Tengah, Semarang, Jawa Tengah, Indonesia ; Telp./Fax: (024)6723014 Email: jlm@poltekkes-smg.ac.id

Statcounter  Lisensi Creative Commons Jaringan Laboratorium Medis disseminated below Creative Commons Attribution-ShareAlike 4.0 International License.

Our Related Accounts :

 

  
MoU PATELKIISSN BRINGoogle ScholarGarudaStat CounterDOI Crossref  

 

Jaringan Laboratorium Medis © 2019