Description of Stability Pooled Plasma CPDA as a Quality Control Material for Uric Acid Examination in Precision Tests with within Run Time and Between Days

Yoki Setyaji, Iin Desmiany Duri, Lilik Setyowatiningsih

Abstract


The quality of laboratory test results is ensured by implementing quality control measures, which usually require control materials. The high cost of commercial control materials poses a challenge for laboratory quality control. Commonly, laboratory control materials are commercial control serums derived from animal serum that undergoes lyophilization during manufacturing. This study aims to assess the stability of CPDA pooled plasma that has been frozen for 60 days, using precision tests both within-run and between-day. Methods: This experimental research utilized CPDA pooled plasma samples from hematology laboratory waste at Poltekkes Kemenkes Semarang. The samples were frozen at -20℃ for 60 days. Quality control (QC) analysis was conducted using within-run and between-day precision tests without replication, to determine the standard deviation (SD) and coefficient of variation (CV). The results were then statistically analyzed using a one-way ANOVA to assess the difference in CPDA pooled plasma stability between the within-run and between-day precision tests. The QC results for within-day and between-day precision were as follows: the average for within-day was 4.066 and for between-day 3.2785; SD for within-day was 0.20 and for between-day 0.09; CV for within-day was 4.9% and for between-day 2.9%. The one-way ANOVA test showed a significance value of 0.316, indicating no significant difference between the within-day and between-day QC methods in terms of precision testing. Conclusion: CPDA pooled plasma remains stable in maintaining the precision of QC results for uric acid using the enzymatic colorimetric method (end-point) after 60-80 days of storage and can be used as an alternative to commercial control materials in uric acid QC testing.




Keywords


CPDA Pooled Plasma ; Precision ; Coefficient of Variation ; Standard Deviation ; Uric Acid QC

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References


Lestari, W. S., Karwiti, W., Latifah, A., Listiani, Y., & Harianja, S. H. (2022). Sera Pooled Stability As a Sgpt Control Material With Storage Time and Temperature Variation. Journal of Medical Laboratory and Science. 2(1): 33–39. Available at: https://doi.org/10.36086/medlabscience.v2i1.1255

Muslim, M., Kustiningsih, Y., & Yanuarti, E. (2021). Pemanfaatan Pool Serum sebagai Bahan Kontrol Ketelitian Pemeriksaan Glukosa Darah. Medical Laboratory Technology Journal. 1(2): 54. Available at: https://doi.org/10.31964/mltj.v1i2.17

Saadah, N.H., Schipperus, M.R., Osselton, J.C.W., Kraaij, M.G.V., Deelder, C.C., Beckers, E.A.M., Leyte, A., Rondeel, J.M.M., de Vooght, K.M.K., Weerkamp, F., Zwaginga, J.J and van der Bom, J.G. (2020). Transition from fresh frozen plasma to solvent/detergent plasma in the Netherlands: comparing clinical use and transfusion reaction risks. Haematologica. 105(4):1158-1165. https://doi.org/10.3324/haematol.2019.222083

Siregar, Maria Tuntun, Doni Setiawan, Anik Nuryati, Wieke Sri Wulan. (2018). Bahan Ajar Teknologi Laboratorium Medik (TLM) Kendali Mutu. Jakarta, Indonesia.

Sujono, Saptono P. dan Ratih H. (2014). Penggunaan Bahan Rujukan Sekunder untuk Validasi Hasil Pemeriksaan Laboratorium Klinik. Jurnal Teknologi Kesehatan volume 10 nomor

https://www.scribd.com/doc/282937398/laboratorium-klinik.

Sukorini, U., Nugroho D. K., Rizki M. dan Hendiawan B. (2010). Pemantapan Mutu Internal Laboratorium Klinik. Yogyakarta: Alfa Media.

Sugiyono. (2019). Metodelogi Penelitian Kuantitatif dan Kualitatif Dan R&D. Bandung: ALFABETA.

Tewabe, H., Mitiku, A., & Yenesew, A. (2024). Validation of the efficacy of Pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory. Practical Laboratory Medicine. 39(March): e00377. Available at: https://doi.org/10.1016/j.plabm.2024.e00377

Tietz, N.W. (1995). Clinical Guide to Laboratory Test, 3th Edition. W.B. Saunders Cp. Philadelphia, PA.

Wicaksono, Muhammad Satrio et al. (2019). Analisa Faktor-Faktor Yang Mempengaruhi Pelaksanaan Quality Control Di Laboratorium. Jurnal Riset Kesehatan Poltekkes Depkes Bandung 11(2): 218–23.

Westgard, James. (2009). What is Multirules Procedure Westgard QC. Tersedia di : https://www.westgard.com

Wulandari, Nada Nabilah, Anik Handayati, and Lully Hanni Endarini. (2023). Stabilitas Serum Kontrol Liofilisat Buatan Sendiri Setelah Rekonstitusi Terhadap Kadar Kolesterol Dan Trigliserida Yang Disimpan Dalam Freezer Suhu (-2°C) Sampai (-4°C) Dan (-20°C). Jurnal Penelitian Kesehatan Suara Forikes 14(4): 67–72. http://forikes-ejournal.com/index.php/SF

Zhang, N., Chen, Q., Zhang, P., Zhou, K., Liu, Y., Wang, H., Duan, S., Xie, Y., Yu, W., Kong, Z., Ren, L., Hou, W., Yang, J., Gong, X., Dong, L., Fang, X., Shi, L., Yu, Y., & Zheng, Y. (2024). Quartet metabolite reference materials for inter ‑ laboratory proficiency test and data integration of metabolomics profiling. Genome Biology. 25(1): 1-21. Available at: https://doi.org/10.1186/s13059-024-03168-z




DOI: https://doi.org/10.31983/jlm.v6i2.10933

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